ABSTRACT
A method for the determination of 15 kinds of nitroaromatics in aqueous samples was developed by dispersive liquid-liquid microextraction and gas chromatography with electron capture detection. A high-density extractant applied in electron capture detector was screened out. The chromatographic conditions, types and dosages of extractants, types and dosages of dispersants, extraction time and the extraction temperature were optimized. The results showed that DB-35 capillary column had the best separation performance for the 15 kinds of nitroaromatics. The nitroaromatics could be separated within 22 min using programmed temperature control as follows: holding at an initial temperature of 80℃ and then heating to 180℃ at a ramping rate of 5℃ / min. For the extraction of 15 kinds of nitroaromatics from 5 mL of aqueous sample, the extraction equilibrium could be reached within 30 s with a high extraction recovery of over 90%when using 100 μL of chlorobenzene as extracting solvent and 400 μL of methanol as disperser solvent. In addition, the enrichment factor could approach a high value of 45. 0 - 48. 8. The sediment collected by centrifugation was injected and analyzed by gas chromatography with electron capture detector. The limits of quantification of the developed method were 0. 03 - 0. 15 μg / L ( S / N = 10). The linear range was from 0. 20 μg / L to 50. 0 μg / L, while the correlation coefficients (R2 ) were more than 0. 998. At the spiked level of 0. 200 μg / L, the relative standard deviations of this method were 3. 3% -8. 9% , the relative recoveries ranged from 86. 0% to 103. 5% . At higher spiked level, the relative standard deviations were less than 5% , and the relative recoveries ranged from 94. 5% to 101. 5% .
ABSTRACT
Objective To evaluate the effect of three methods on detecting cleaning efficacy of suction luminal de-vices in pediatric department,and find out a proper detection method for such devices.Methods Random number table method was adopted to take samples of suction lumen devices after cleaned in central sterile supply department in January-April 2016,visual observation with the aid of magnifying glass,jieli strip detection method,and ATP bi-oluminescence assay were adopted to detect cleaning efficacy of outer surface,screwed connection,and inner wall of lumen devices,the qualified rates of cleaning detected by three methods were compared.Results The qualified rates of suction lumen devices after cleaned detected by visual observation with the aid of magnifying glass,jieli strip de-tection method,and ATP bioluminescence assay were 99.00%,91.00%,and 71.50% respectively,qualified rates of cleaning detected by three methods were statistically significant(χ2 =71.545,P <0.001),pairwise comparison showed a significant difference (both P <0.0167);qualified rates of cleaning of the outer surface of lumen detected with three methods were all 100.00%;qualified rates of cleaning of screwed connection were 100.00%,98.00%, and 97.00% respectively(χ2 =5.695,P >0.05);qualified rates of cleaning of inner wall were 99.00%,92.00%, and 72.00% respectively(χ2 = 72.634,P < 0.01 ),pairwise comparison were all significantly different(all P <0.01).Conclusion The cleaning quality of the outer surface and screwed connection of pediatric suction lumen de-vices can be assessed by visual observation with the aid of magnifying glass,cleaning quality of the inner wall of lu-men can be detected by ATP bioluminescence method,when condition is limited,Jieli strip method can be used for routine detection,supplemented by ATP bioluminescence detection on a regular basis.
ABSTRACT
Objective To evaluate the effect of three methods on detecting cleaning efficacy of suction luminal de-vices in pediatric department,and find out a proper detection method for such devices.Methods Random number table method was adopted to take samples of suction lumen devices after cleaned in central sterile supply department in January-April 2016,visual observation with the aid of magnifying glass,jieli strip detection method,and ATP bi-oluminescence assay were adopted to detect cleaning efficacy of outer surface,screwed connection,and inner wall of lumen devices,the qualified rates of cleaning detected by three methods were compared.Results The qualified rates of suction lumen devices after cleaned detected by visual observation with the aid of magnifying glass,jieli strip de-tection method,and ATP bioluminescence assay were 99.00%,91.00%,and 71.50% respectively,qualified rates of cleaning detected by three methods were statistically significant(χ2 =71.545,P <0.001),pairwise comparison showed a significant difference (both P <0.0167);qualified rates of cleaning of the outer surface of lumen detected with three methods were all 100.00%;qualified rates of cleaning of screwed connection were 100.00%,98.00%, and 97.00% respectively(χ2 =5.695,P >0.05);qualified rates of cleaning of inner wall were 99.00%,92.00%, and 72.00% respectively(χ2 = 72.634,P < 0.01 ),pairwise comparison were all significantly different(all P <0.01).Conclusion The cleaning quality of the outer surface and screwed connection of pediatric suction lumen de-vices can be assessed by visual observation with the aid of magnifying glass,cleaning quality of the inner wall of lu-men can be detected by ATP bioluminescence method,when condition is limited,Jieli strip method can be used for routine detection,supplemented by ATP bioluminescence detection on a regular basis.